Characterization of denaturation and renaturation of DNA for DNA hybridization
نویسندگان
چکیده
منابع مشابه
Characterization of denaturation and renaturation of DNA for DNA hybridization
OBJECTIVES The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. METHODS A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was de...
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Here we report a thermodynamic analysis of biomolecular interactions using MicroScale Thermophoresis (MST). Using DNA hybridization with fluorescently labeled oligonucleotides as a well-characterized model system, we monitor the shift in the dissociation (Kd) constant of the interaction over a range of temperatures and calculate the free enthalpy (ΔH) and entropy (ΔS) for the hybridization reac...
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* Supported in part by a research contract NONR 266 (63) between the Office of Naval Research and Columbia University, and by a grant-in-aid from the Bristol-Myers Company. t Postdoctoral Research Fellow of the National Institute of Dental Research. Present address: National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland. t Present address: School of Dentistry, ...
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ژورنال
عنوان ژورنال: Environmental Health and Toxicology
سال: 2014
ISSN: 2233-6567
DOI: 10.5620/eht.2014.29.e2014007